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P2938
Sigma
Immobilon®-P Polyvinylidene Difluoride membranes
size 26.5 cm × 3.75 m
| Synonym: | PVDF |
Description
| Application | Polyvinylidene difluoride membranes are popular membranes for immunoblotting (Western blotting),1 and sequencing2 of proteins. The basis of protein binding to PVDF is believed to be due to hydrophobic interactions. PVDF membranes for Western blotting are available in the most commonly used 0.45 μm pore size. PVDF membranes have a greater protein binding capacity, are more durable and more solvent resistant than nitrocellulose. |
| Legal Information | Immobilon is a registered trademark of Millipore Corp. |
Properties
| size | 26.5 cm × 3.75 m |
Safety
| Personal Protective Equipment | Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter |
| WGK Germany | 3 |
References
| Cited Reference | 1. Pluskal, M.G., et al., Immobilon PVDF transfer membrane: A new membrane substrate for Western blotting of proteins. Biotechniques 4, 272-282, (1986) |
| 2. Hirano, H., et al., Microsequence analysis of the N-terminally blocked proteins immobilized on polyvinylidene difuoride membrane by Western blotting. Electrophoresis 14, 839-846, (1993) | |
| reference | Suck, R.W.L. and Krupinska, K., Repeated probing of Western blots obtained from Coomassie Brilliant blue-stained or unstained polyacrylamide gels. Biotechniques 21, 418-422, (1996) |
